relationship of sperm parameters with lipid peroxidation in asthenozoospermic and normozoospermic males

نویسندگان

heidar tavilani department of medical biochemistry, hamadan university of medical sciences, hamadan,

mahmoud doosti department of medical biochemistry, faculty of medicine, tehran university of medical sciences

hojatollah saeidi , the department of medical pathology, tehran university of medical sciences, tehran,سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (tehran university of medical sciences)

seyed mohammad hosseinipanah he department of anatomy, kashan university of medical sciences, kashan, i.r. iran.

چکیده

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with motility grades: a (rapid progressive), b (slow progressive), c (nonprogressive ), d (immotile ), percent of normal morphology, acrosome and tail defect, and concentration of spermatozoa was determined. methods: mda of spermatozoa and seminal plasma was determined in 35 asthenozoospermic and 15 normozoospermic men by spectrofluorometry. semen analysis was done according to the who standard. results: mda in the spermatozoa of asthenozoospermic patients was significantly higher than normozoospermic males (0.14±0.0 1 and 0.09±0.0 1 nmol/1 0* 106 spermatozoa respectively, mean ±se). a significant negative correlation was observed between mda of spermatozoa with grade a motility and concentration of spermatozoa. in addition, a significant positive correlation was observed between mda of spermatozoa with the acrosome and tail defect. the mda value in the seminal plasma of asthenozoospermic and normozoospermic patients was 1.35±0.07 and 1.2±0.08 nmol/ml seminal plasma respectively (mean±se). the mda of seminal plasma exhibited a negative correlation with grade a+b motility and positive correlation with grade c+d motility and head defect. conclusion: lipid peroxidation has a deleterious effect on semen quality and mda is an index of lipid peroxidation which may be a diagnostic tool for the analysis of infertility in asthenozoospermic patients.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

RELATIONSHIP OF SPERM PARAMETERS WITH LIPID PEROXIDATION IN ASTHENOZOOSPERMIC AND NORMOZOOSPERMIC MALES

Background: The lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. The purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. In addition, correlation of lipid peroxidation with mot...

متن کامل

Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples.

Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Androlo...

متن کامل

Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies

Recent studies have shown that infertility affects estimated 15% of all couples. Male infertility is the primary or contributory cause in 60% of these cases. Consequently, the application of assisted reproduction is increasing. These methods could benefit from an extended evaluation of sperm quality. For this reason, we analyzed sperm proteins from 30 men with normal spermiograms and 30 men wit...

متن کامل

[Evaluation of sperm functional parameters in normozoospermic infertile individuals].

OBJECTIVE This study evaluated the integrity of the chromatin structure, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), DNA damage and lipid peroxidation of semen samples from infertile men classified as unexplained infertility. METHODS Between February 2010 and July 2011 semen parameters and functional tests were evaluated in 10 subjects with proven fertility, ...

متن کامل

Vitamins C, E and lipid peroxidation levels in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men

Background: It has been shown that reactive oxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. Vitamins E and C are capable of reducing ROS levels and lipid peroxidation. Objective: The aim of study was to determine the level of lipid peroxidation as indicated by Malondialdehyde (MDA) and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic a...

متن کامل

Role of total antioxidant capacity and lipid peroxidation in fertile and infertile men

Aim of the present study is to investigate the total antioxidant capacity (TAC) and lipid peroxidation end product, malondialdehyde (MDA) concentration in seminal plasma of fertile and infertile men and its relationship with different spermatozoa parameters. Semen samples were collected from 15 fertile men (normozoospermic as control) and 40 infertile men (15 oligozoospermic, 17 asthenozoosperm...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۹، شماره ۴، صفحات ۳۳۹-۳۴۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023